1.
Quality Evaluation Of Different Brands Of Ceftriaxone
by Sana Tariq | Prof. Dr. Muhammad Ashraf | Dr. Muhammad Adil Rasheed | Miss Huma.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2012Dissertation note: This study was designed to determine the physicochemical equivalence of selected brands of ceftriaxone sodium registered with the ministry of health government of Islamic republic of Pakistan. Out of 9 selected brands 3 were of lowest price distribution class, 3 from the intermediate price distribution class and remaining three from the highest price distribution class. For quality evaluation 3 parameters were selected which were physical, chemical and microbial. Physical characters analyzed were general appearance, pH, solubility and moisture content. Characters which determine the chemical equivalence were assay of active ingredient and percentage of impurities present in powdered drug. Both these parameters were quantified chromatographically using high pressure liquid chromatography. Clinical efficacy of selected brands of this valuable antibiotic was accessed by determining the minimal inhibitory concentrations against Staphylococcus aureus, Salmonella typhi, Klebsiella pneumonia and Escherichia coli.
Statistically all brands were significantly different from one another but all the parameters taken as quality indicators showed results within the range specified by united state pharmacopoeia.
None of selected brands of ceftriaxone sodium were found to be counterfeit or even substandard. Irrespective of difference in price, no visible variation was found among different quality assessment parameters, all samples showed compliance with the international pharmacopoeial standards. Through this study it can be concluded that the quality of ceftriaxone in Pakistan is well regulated, all the registered brands are up to the mark and irrespective of variation in price there is no variation in the quality of brands.
Availability: Items available for loan: UVAS Library [Call number: 1462,T] (1).
2.
Chemical Equivalence Of Different Brands Of Oxytertacycline Hydrochloride And Its Minimum
by Sadaf Hina | Prof. Dr. Muhammad Ashraf | Dr. Aftab | Dr. Muhammad Adil Rasheed.
Material type: ; Format:
print
; Nature of contents: ; Literary form: Publisher: 2012Dissertation note: This project was designed to study the chemical equivalence of various brands of Oxytetracycline hydrochloride (long acting, short acting & PVP) approved by the ministry of health and available in the local market for veterinary use. Oxytetracycline was measured by HPLC method developed and standardized in the laboratory. Limit of detection (LOD) and limit of quantification (LOQ) of the Oxytetracycline by HPLC assay method were determined. From stock solution of working standard (Oxytetracycline hydrochloride) different concentrations 0.05, 0.1, 0.5, 1.0, 10, 25, 50 and 100µg per ml were prepared for the determination of LOD. The LOD calculated was 0.100(µg/ml) and LOQ was 0.5 (µg/ml). Correlation coefficient was 0.99994050. Concentration of the active ingredient (Oxytetracycline hydrochloride) in all preparations was same as mentioned on the label except Oxytetracycline (74%), Terrasym PVP-100 (81%), and Onyx-LA (72%).
MIC of Oxytetracycline hydrochloride against following bacterial isolates determined by micro-broth dilution test was Bacillus subtilis (50µg), Staphylococcus aureus (100µg), Eschericiha coli (50µg), Salmonella enterica (1000µg) and Pasturella multocida (50µg).It showed that all these bacterial cultures have developed resistance against Oxytetracycline hydrochloride.
Availability: Items available for loan: UVAS Library [Call number: 1468,T] (1).
3.
Pharmaceutical Equivalence Of Different Brands Of Moxifloxacin Hydrochloride And Minimum Inhibitory Concentration
by Sarmat Tamjeed Afzal | Dr. Muhammad Adil Rasheed | Prof. Dr. Muhammad Ashraf.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2012Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1471,T] (1).
4.
Mutagenic And Cytotoxic Evaluartion Of Piroxicam And Meloxicam
by Snober Khatoon Akram | Prof. Dr. Muhammad Ashraf | Dr. Muhammad Adil Rasheed | Dr.Aftab | Faculty of Bio-Sciences.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Piroxicam and Meloxicam are enolic acid derivatives and belong to oxicam class of non steroidal anti-inflammatory drugs. They are therapeutically used in rheumatoid arthritis and osteoarthritis. This study was designed to evaluate mutagenicity and cytotoxicity of piroxicam and meloxicam by Ames Salmonella/microsome mutagenicity assay and MTT assay. In this study, ten concentrations (100µg/ml, 300µg/ml, 500µg/ml, 700µg/ml, 900µg/ml, 1000µg/ml, 3000µg/ml, 5000µg/ml, 7000µg/ml and 10,000µg/ml) of piroxicam and meloxicam were used in Ames test against Salmonella strain TA100 in plate incorporation method, with and without metabolic activation S-9 mixture in triplicate manner. In MTT assay, confluent monolayer of BHK-21 cell lines was used and grown in 96-well cell culture plates treated with same concentrations of both drugs in triplicate manner. The results indicated that piroxicam had no mutagenic potential at concentrations of 100µg/plate to 3000µg/plate, possible mutagenic potential at 5000µg/plate and significant mutagenic potential at concentration of 7000µg/plate and 10,000µg/plate. Meloxicam had no mutagenic potential at the concentrations 100µg/plate to 7000µg/plate and possible mutagenic potential at highest concentration 10,000µg/plate. The cytotoxic effect of piroxicam and meloxicam at the concentrations of 100µg/ml to 5000µg/ml was none cytotoxic and at the concentration of 7000µg/ml and 10,000µg/ml cytotoxic to BHK-21 cell lines. There was significant increased in mutant frequency with increased in concentration of both drugs with and without metabolic activation S-9 mixture. There was significant difference in non mutagenic, possible mutagenic and significant mutagenic potential doses of piroxicam. There was no significant difference in none cytotoxic doses of both drugs. In comparison of both drugs, there was no significant difference in mutagenicity and cytotoxicity. It concluded that piroxicam and meloxicam were not mutagenic and cytotoxic at therapeutic doses. Piroxicam had mutagenic potential in dose dependent manner. Both drugs were cytotoxic at higher concentrations. They had same cytotoxic effect in dose dependent manner.
Availability: Items available for loan: UVAS Library [Call number: 1548,T] (1).
5.
Evaluation And Comparison Of Anti-Viral Activity Of Ethanolic And Chloroformic Extract Of Juniperus Excelsa Against Peste Des Petits Ruminants Virus
by Amber Sharif | Prof. Dr. Muhammad Ashraf | Dr. Muhammad Adil Rasheed | Mr. Allah.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1723,T] (1).
6.
Efficacy Of Anti-Microbial Agents Withascorbic Aci In Catheter Associated Urinary Tract Infection
by Sana Afzal | Prof. Dr. Muhammad Ashraf | Dr. Muhammad Adil Rasheed | Malik Allah.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Catheter-associated urinary tract infections (CAUTIs) are most common type of nosocomial infection. This study was designed to recognize and identify the bacterial pathogen of catheter-associated urinary tract infection in patients, the sensitivity pattern of bacterial pathogens to commonly used antibiotics and the synergistic effect of antibiotics with ascorbic acid to improve the efficacy of antibiotics. The prospective, observational study was conducted in Sir Ganga Raam hospital Lahore. The urine samples from 100 catheterized patients were collected and were analyzed for its causative /pathogenic organism. Out of 100 patients, 58 patients carried E.coli (27%), Citrobacter (22%), Enterobacter (5%) and Staphylococcus aureus (4%) and these patients were included in study. Sensitivity patterns of Ampicillin, Co-amoxiclav, Ceftriaxone and Ciprofloxacin were checked by Kirby-Bauer disk diffusion method. Uropathogens appeared to be highly resistant against Ceftriaxone (84%), followed by Co-amoxiclave (83%) and Ampicillin (76%).Amikacin and Ciprofloxacin are effective drugs against uropathogens and their sensitivities to Amikacin and Ciprofloxacin were 74% and 71% respectively.
Susceptibility testing of bacteria against antibiotics and ascorbic acid alone and in combination was checked and it was observed that bacterial resistance to Ceftriaxone was reversed with ascorbic acid and the effectiveness of ciprofloxacin was improved with ascorbic acid. In Citrobacter, ascorbic acid antagonized the effects of Amikacin.
Empirical therapy of patients included in study was evaluated by clinical response and their definitive therapy was assessed by observing the adverse effects associated with that drug. Co-amoxiclav produced 100% side effects. Tinnitus (63%) was observed in patients treated with Amikacin while Ciprofloxacin adverse effects were headache and dizziness.
It was concluded in the study that there was high incidence of infection in catheterized patients with resistant bacteria and inappropriate antibiotic therapy. Ascorbic acid may be prescribed prophylactically to catheterized patients and to those who take Ceftriaxone to minimize its resistance in patients. To improve the effectiveness of drugs in CAUTI patients, ascorbic acid may be used with antibiotics.
Availability: Items available for loan: UVAS Library [Call number: 1794,T] (1).
7.
Genotoxic and Mutagenic Potential of Anti-Diabetic Drugs (Sitagliptin and Metformin) Alone And In Combination With Artificial Sweeteners.
by Komal Najam | Prof. Dr. Muhammad Ashraf | Dr. Imran Altaf | Dr. Muhammad Adil Rasheed.
Material type: ; Format:
print
Publisher: 2013Dissertation note: Metformin most commonly prescribed oral anti hyperglycemic drug for type 2 diabetes whereas Sitagliptin recently approved oral antidabetic drug for type 2 diabetes were evaluated for their mutagenic and genotoxic potential alone and in combination with three artificial sweeteners (Saccharin, Aspartame and Acesulfame-K) normally consumed by diabetic individuals.
In this research project Ames Salmonella/microsome assay was performed to check the mutagenicity of Metformin and Sitagliptin alone and in combination with artificial sweeteners using mutant Salmonella tester strains TA100 and TA98 with and without the S9 whereas Genotoxicity was evaluated by Single Cell Gel Alkaline Electrophoresis/Comet.
The results indicated that Metformin alone showed mutagenic effect at 120µg/plate against TA100 with S9mix. However Metformin when tested in combination with artificial sweeteners, significant enhance in mutagenicity occurred only against TA100 with and without S9. Sitagliptin displayed mutagenic potential only to TA98 with S9mix at the concentration of 3040µg/plate. In addition significant enhance in mutagenicity occurred when tested in combination with artificial sweeteners against both strains with and without S9. In case of genotoxicity both Metformin and Sitagliptin results indicated significant increase in DNA damage in dose dependant manner as compared to negative control. Though Metformin and Sitagliptin in combination with artificial sweetener did not reveal any significant boost in the genotoxicity relative to when they were tested alone.
Availability: Items available for loan: UVAS Library [Call number: 1799,T] (1).
8.
Effect Of Aqueous Extract Of Leaves Of Acacia Nilotica On Angiogenesis.
by Muhammad Yasin | Dr. Muhammad Adil Rasheed | Prof. Dr. Muhammad Ashraf.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1803,T] (1).
9.
Reversal Of Antibiotics Resistance In Methicillin-Resistant Staphylococcus Aureus By Non-Antimicrobial Agents
by Sohaib Danyaal | Prof. Dr. Muhammad Ashraf | Dr. Aftab | Dr. Muhammad Adil Rasheed.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2014Dissertation note: Antibiotic resistance is increasing along with the increasing use of antibiotic for the treatment of infectious diseases. The in-vitro study was designed to observe the reversal of
antibiotics resistance in Methicillin-resistant Staphylococcus aureus by non-antimicrobial
agents.
One hundred pus samples were processed for isolation and identification of MRSA.
Out of 100, 37 (37%) islotes were Gram +ve cocci, from these 37 isolates 34 (92%) turn red
colour of mannitol salt media to yellow and 23 (62%) gave +ve catalase and coagulase. In
this study Antibiotic Sensitivity Test was performed on pure culture of MRSA strain by
applying disc diffusion method. Out of 23 pure MRSA isolate, 100% isolates were
methacillin resistant, 79% isolates were co-amoxiclav resistant, 30% isolates were
meropenum resistant, 8% isolates were vancomycin resistant, 26% isoltes were moxifloxacin
resistant and 39% isolates were linezolid resistant. Reversal of antibiotics resistance was
observed by MIC or serial dilution method, using non antibiotic agents like Amiloride,
Lansoprazole and Promethazine, Concentrations of non-antibiotic agents 1024ìg, 512ìg,
256ìg, 128ug, 64ìg and 32ìg were used in combination of antibiotics to reverse the
antibiotics resistance in MRSA. These non antibiotic agents may cause the alteration in
mechanisms by which microorganism develop resistance. The collected data analyzed by
applying analysis of variance (ANOVA) through SPSS 16.0 computer software. Now we would be
able to treat some lethal infection caused by MRSA, and help to increase patient compliance
and decrease the cost of therapy.
Availability: Items available for loan: UVAS Library [Call number: 1828,T] (1).
10.
Role Of Non-Antimicrobial Agents In Reversal Of Antibiotic Resistance In Escherichia Coli
by Kalim Ullah | Prof. Dr. Muhammad Ashraf | Dr. Muhammad Adil Rasheed | Prof. Dr. Aftab.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1839,T] (1).
11.
Evaluation Of Antimicrobial Therapy In Neonatal Sepsis
by Shazia Khanum | Prof. Dr. Muhammad Ashraf | Dr. Muhammad Adil Rasheed | Mr. Allah.
Material type: ; Format:
print
; Literary form:
drama
Publisher: 2013Dissertation note: Background: Sepsis is the presence of bacteria in blood. Neonatal sepsis indicates bacterial blood stream infection (BSI), such as gastroenteritis meningitis, pyelonephritis, pneumonia which results in fever in neonates (Weber et al. 2003). This study was designed to check the bacteriology and sensitivity pattern of microbes causing sepsis in neonates. In this study microbial susceptibility testing against antibiotics and pattern of resistance of microbes in septic patients was determined. It was conducted on neonates suspected from sepsis in Sir Ganga Ram Hospital Lahore, Pakistan. Hypothesis: By using blood cultures and different biochemical tests bacterial etiological agents of neonatal sepsis were determined and their sensitivity pattern and empirical therapies were evaluated. Material & Methods: Blood samples were collected from septic neonates admitted in Sir Ganga Ram Hospital keeping all necessary aseptic precautions with the assistance of trained professionals. The pathogens were isolated, identified and purified by selective culturing methods, which were subjected to active growth, during which sensitivity to different antibiotics were checked. The sensitivity was measured by area marked by the zone of inhibition, and National Committee for Clinical Laboratory Standards (NCCLS). Standard limit was a key indicator towards resistant bacteria. Statistical Analysis: The collected data was analyzed by appropriate statistical procedure. Outcome: It was designed to isolate and identify the pathogens responsible for neonatal sepsis and to see the effects of different antibiotics regimens for treatment of neonatal sepsis by evaluating the improvement in clinical condition, rate of complications of disease and incidence of death due to this fatal disease.
Availability: Items available for loan: UVAS Library [Call number: 1843,T] (1).
12.
Comparative Mutagenic And Cytotoxic Evaluation Of Tamsulosin And Ciprofloxacin
by Faiza qamar | Dr. Muhammad Adil rasheed | Prof. Dr, Muhammad Ashraf.
Material type: ; Format:
print
Publisher: 2013Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 1875,T] (1).
13.
Evaluation Of Antibacterial Activity Of Macrolides In Combination With Levamisole Hc1 Against Different Pathogenic Bacteria
by Sheeza javaid | Prof. Dr. Muhammad Ashraf | Dr. Muhammad Adil rasheed | Proff. Dr. Aftab.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2026,T] (1).
14.
Bioequivalence Study Of Simvastatin In Healthy Human Volunteers
by Rana muhammad wasim shahzad | Dr. Muhammad adil rasheed | Prof. Dr. Muhammad ashraf.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2044,T] (1).
15.
Effect Of Calcium Hypochlorite And Chloramine On Blood Biochemistry And Sodium Pentobarbital Induced Sleeping
by Sidra ishaq | Dr. Muhammad Adil rasheed | Prof. Dr. Muhammad Ashraf.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2062,T] (1).
16.
Antibacterial And Cytotoxic Evaluation Of Aqueous Ethanolic And Chlorooform Extracts Of Parthenium Hysterophorus
by Sidrah Shoaib | Dr. Muhammad Adil Rasheed | Prof. Dr. Muhammad Ashraf.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2109,T] (1).
17.
Synergistic Effect Of Antimicrobial Agents In Combination With Colistin Against Bacterial Isolates From Patients
by Sahar Safdar | Prof. Dr. Muhammad Ashraf | Dr. Muhammad Adil Rasheed | Prof. Dr. Aftab.
Material type: ; Format:
print
; Literary form:
not fiction
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2123,T] (1).
18.
Evaluation Of Mutagenicity And Genotoxicity Of Furosemide And Propranolol Alone And In Combination Using Ames Test And Single Cell Gel Electrophoresis (Comet) Assay
by Sadaf Naz | Dr. Muhammad Adil rasheed | Prof. Dr. Muhammad Ashraf.
Material type: ; Format:
print
Publisher: 2014Dissertation note: Abstract Availability: Items available for loan: UVAS Library [Call number: 2158,T] (1).
